Journal: Viruses

Article Title: Optimisation of Neuraminidase Expression for Use in Drug Discovery by Using HEK293-6E Cells

doi: 10.3390/v13101893

Figure Lengend Snippet: NA crystallization and X-ray diffraction. ( a ) Crystals in a hit condition containing 0.1 MES monohydrate pH 6.5, 12% w/v PEG-20,000. ( b ) Flat square plates (~100 µm × 100 µm × 5 µm) resulted from optimisation of hit condition. ( c ) X-ray diffraction image produced by produced by a H1N1 NA crystal collected at the MX2 beamline at the Melbourne Synchrotron. Maximum resolution of 2.04 Å at the edge of the detector, beam energy 12,664.9 eV, detector distance 300 mm, exposure time 1 s. A larger version of this diffraction image can be found in .

Article Snippet: The crystal screens tested were Wizard I and II random sparse matrix crystallization screens (Emerald BioSystems, Bainbridge Island, WA, USA), and Crystal ScreenTM HR2-110 and HR2-112 (Hampton Research, CA, USA).

Techniques: Crystallization Assay, Produced